Microheterogeneity of S-glycoprotein of mouse hepatitis virus temperature-sensitive mutants. uri icon

abstract

  • Mouse hepatitis virus (MHV) strain JHM (MHV-JHM) is a neurotropic coronavirus that causes acute fatal encephalomyelitis in 75-99% of infected mice. The surviving animals may subsequently develop demyelinating disease. We compared the S peplomer protein of the wild type (wt) and five temperature-sensitive (ts) mutants of MHV-JHM. In contrast with the wt, none of these five cause fatal disease (mortality less than 10%). Three of these ts mutants did not induce any demyelinating disease, a fourth caused demyelinating disease in 5% of the animals and a fifth, designated ts8, exhibited strong demyelinating properties and caused demyelination in 99% of the animals. SDS-PAGE analysis revealed no differences in the molecular weight of S peplomer protein of wt or ts MHV-JHM mutants. However, isoelectric focusing of the S protein of these five ts mutants and the wt MHV-JHM, followed by transfer to nitrocellulose sheets and immunoblotting with anti-S specific antibody revealed significant differences in the microheterogeneity of the S protein.

published proceedings

  • J Virol Methods

author list (cited authors)

  • Oleszak, E. L., Knisley, K., Rodkey, L. S., & Leibowitz, J. L.

citation count

  • 2

complete list of authors

  • Oleszak, EL||Knisley, K||Rodkey, LS||Leibowitz, JL

publication date

  • July 1992