MHV-3 induced prothrombinase is encoded by musfiblp. Academic Article

abstract

• Previously, we demonstrated induction of a unique macrophage prothrombinase, PCA, in MHV-3 infected BALB/cJ mice. By immunologic screening, a clone representing PCA was isolated from a cDNA library and sequenced. The sequence identified this clone as representing part of a gene, musfiblp, that encodes a fibrinogen-like protein. Six additional clones were isolated, and one clone, p11-3-1, encompassed the entire coding region of musfiblp. Murine macrophages did not constitutively express musfiblp, but when infected with MHV-3, synthesized musfiblp-specific mRNA. Musfiblp mRNA induction was earlier and significantly greater in BALB/cJ than A/J macrophages. Prothrombinase activity was demonstrate when musfiblp was expressed from p11-3-1 in RAW 264.7 cells. These data suggest that musfiblp encodes the MHV-induced prothrombinase.

authors

• Leibowitz, Julian

published proceedings

• Adv Exp Med Biol

author list (cited authors)

• Parr, R. L., Fung, L., Reneker, J., Myers-Mason, N., Leibowitz, J. L., & Levy, G.

citation count

• 5

complete list of authors

• Parr, RL||Fung, L||Reneker, J||Myers-Mason, N||Leibowitz, JL||Levy, G

publication date

• January 1995

publisher

• Springer Nature  Publisher

published in

• Advances in Experimental Medicine and Biology  Journal

keywords

• Animals
• Blood Coagulation Factors
• DNA, Complementary
• Enzyme Induction
• Female
• Gene Expression Regulation, Viral
• Gene Library
• Macrophages
• Mice
• Mice, Inbred A
• Mice, Inbred BALB C
• Murine Hepatitis Virus
• Species Specificity
• Thromboplastin

PubMed Central ID

• 8830472

Digital Object Identifier (DOI)

• 10.1007/978-1-4615-1899-0_25

start page

• 151

end page

• 157

volume

• 380

URL

• http://dx.doi.org/10.1007/978-1-4615-1899-0_25