Babesia bovis: Gene isolation and characterization using a mung bean nuclease-derived expression library Academic Article uri icon

abstract

  • Genomic DNA prepared from erythrocyte cultures of Babesia bovis merozoites was digested with mung bean nuclease and used to construct a gt11 expression library of B. bovis recombinants. Immunoscreening with two polyclonal antibody probes detected multiple recombinants from which two, designated Bb-1 and Bb-3, were chosen for further analysis. Monospecific immunoglobulins isolated from the screening sera using nitrocellulose-bound fusion proteins were employed to determine the native molecular weight and the intracellular location of the babesial proteins encoded by the recombinants. Clone Bb-1 encodes an antigen of 77,000 Da located at the apical end of the intraerythrocytic parasite. A protein of 75,000 Da encoded by clone Bb-3 is associated with the infected red blood cell cytoplasm and/or membrane but not with the merozoite. 1989.

published proceedings

  • Experimental Parasitology

altmetric score

  • 6

author list (cited authors)

  • Tripp, C. A., Wagner, G. G., & Rice-Ficht, A. C.

citation count

  • 30

complete list of authors

  • Tripp, CA||Wagner, GG||Rice-Ficht, AC

publication date

  • January 1, 1989 11:11 AM