Detection of bacterial infection with a fiber optic microendoscope Conference Paper uri icon

abstract

  • We present the use of fiber optic microendoscopy to image bacterial infection in the skin and lungs using an animal model. The contact probe microendoscope we have constructed has a 4 μm resolution, a 750 μm field of view, and a 1 mm outer diameter. Well resolved regions of bacterial infection were imaged for subcutaneous inocula of 10 6 to 10 1 CFU and intra-tracheal inocula of 10 8 to 10 6 CFU. Results reveal a linear relationship between average fluorescence and CFU, suggesting potential for using this device for quantitative analysis. Detection limits of 10 4 CFU for skin samples and 10 7 CFU for lung tissue were determined. In addition, bacteria were also qualitatively visible in lung tissue down to 10 6 CFU. Confocal imaging was used to confirm the presence of bacteria in tissue samples. © 2011 Copyrigt Society of Photo-Optical Instrumentation Engineers (SPIE).

name of conference

  • European Conference on Biomedical Optics

published proceedings

  • Medical Laser Applications and Laser-Tissue Interactions V

author list (cited authors)

  • Mufti, N., Kong, Y., Cirillo, J. D., & Maitland, K. C.

citation count

  • 0

complete list of authors

  • Mufti, Nooman||Kong, Ying||Cirillo, Jeffrey D||Maitland, Kristen C

publication date

  • January 2011

publisher

  • OSA  Publisher