Detection of bacterial infection with a fiber optic microendoscope
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abstract
We present the use of fiber optic microendoscopy to image bacterial infection in the skin and lungs using an animal model. The contact probe microendoscope we have constructed has a 4 m resolution, a 750 m field of view, and a 1 mm outer diameter. Well resolved regions of bacterial infection were imaged for subcutaneous inocula of 10 6 to 10 1 CFU and intra-tracheal inocula of 10 8 to 10 6 CFU. Results reveal a linear relationship between average fluorescence and CFU, suggesting potential for using this device for quantitative analysis. Detection limits of 10 4 CFU for skin samples and 10 7 CFU for lung tissue were determined. In addition, bacteria were also qualitatively visible in lung tissue down to 10 6 CFU. Confocal imaging was used to confirm the presence of bacteria in tissue samples. 2011 Copyrigt Society of Photo-Optical Instrumentation Engineers (SPIE).
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Medical Laser Applications and Laser-Tissue Interactions V