Detection of bacterial infection with a fiber optic microendoscope
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Overview
abstract
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We present the use of fiber optic microendoscopy to image bacterial infection in the skin and lungs using an animal model. The contact probe microendoscope we have constructed has a 4 μm resolution, a 750 μm field of view, and a 1 mm outer diameter. Well resolved regions of bacterial infection were imaged for subcutaneous inocula of 10 6 to 10 1 CFU and intra-tracheal inocula of 10 8 to 10 6 CFU. Results reveal a linear relationship between average fluorescence and CFU, suggesting potential for using this device for quantitative analysis. Detection limits of 10 4 CFU for skin samples and 10 7 CFU for lung tissue were determined. In addition, bacteria were also qualitatively visible in lung tissue down to 10 6 CFU. Confocal imaging was used to confirm the presence of bacteria in tissue samples. © 2011 Copyrigt Society of Photo-Optical Instrumentation Engineers (SPIE).
name of conference
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European Conference on Biomedical Optics
published proceedings
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Medical Laser Applications and Laser-Tissue Interactions V
author list (cited authors)
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Mufti, N., Kong, Y., Cirillo, J. D., & Maitland, K. C.
citation count
complete list of authors
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Mufti, Nooman||Kong, Ying||Cirillo, Jeffrey D||Maitland, Kristen C
publication date
publisher
Research
keywords
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Bacterial Imaging
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Endoscopic Imaging
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Fiber Optics Imaging
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Fluorescent Bacteria
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Macroscopic Imaging
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Microendoscopy
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Tuberculosis
Identity
Digital Object Identifier (DOI)
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