Effect of ionic strength and temperature on the protonation of oxidized glutathione uri icon

abstract

  • The protonation constants for oxidized glutathione, Hi-1L (4-i+1)-, KiH =[HiL (4-i)-]/[Hi-1L(4-i+1)-][H+] i=1,2,...,6 have been measured at 5, 25 and 45C as a function of the ionic strength (0.1 to 5.4 mol[kg(H2O)]-1) in NaCl solutions. The effect of ionic strength on the measured protonation constants has been used to determine the thermodynamic values (KiH0 ) and the enthalpy (Hi ) for the dissociation reaction using the SIT model and Pitzer equations. The SIT () and Pitzer parameters ((0), (1) and C) for the dissociation products (L4-, HL3-, H2L2-, H 3L-, H4L, H5L+, H 6L2+) have been determined as a function of temperature. These results can be used to examine the effect of ionic strength and temperature on glutathione in aqueous solutions with NaCl as the major component (body fluids, seawater and brines). 2008 Springer Science+Business Media, LLC.

published proceedings

  • JOURNAL OF SOLUTION CHEMISTRY

author list (cited authors)

  • Crea, P., De Stefano, C., Kambarami, M., Millero, F. J., & Sharma, V. K.

citation count

  • 7

complete list of authors

  • Crea, Pasquale||De Stefano, Concetta||Kambarami, Mutsa||Millero, Frank J||Sharma, Virender K

publication date

  • September 2008