A LC-MS/MS CONFIRMATORY METHOD FOR DETERMINATION OF CHLORAMPHENICOL IN REAL SAMPLES SCREENED BY COMPETITIVE IMMUNOASSAY
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A new liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed to confirm chloramphenicol (CAP) residues in foods of animal origin and in urine samples, which were earlier found positive under the screening analysis, performed by competitive enzyme-linked immunoassay (ELISA) technique. The developed LC-MS/MS method was applied to four non-compliant samples from 2008 to 2012; giving concentrations of CAP residues from 1.18 to 3.68 g kg-1. All samples, qualified positive by ELISA, were confirmed with the LC-MS/MS technique and found to be non-compliant. The effectiveness of the confirmatory method was proven by participating in a successful proficiency test in year 2010. Both LC-MS/MS and ELISA methods were validated according to the European Union 2002/657/EC decision. The decision limit of the confirmatory method was determined as 0.02 g kg-1 for CAP in each validated matrix, while the detection capability of the screening test was 0.15 g kg-1.
author list (cited authors)
Toelgyesi, A., Fekete, J., Sharma, V. K., Palffi, E., Bekesi, K., Lukonics, D., & Pleva, G.
complete list of authors
Toelgyesi, A||Fekete, J||Sharma, VK||Palffi, E||Bekesi, K||Lukonics, D||Pleva, G