A SIMPLE METHOD FOR DIFFERENTIAL ASSAY OF TRITIUM AND CARBON-14 IN WATER-SOLUBLE BIOLOGICAL MATERIALS.

abstract

• A methyl Cellosolve-toluene phosphor solvent system is described for the differential determination of absolute levels of tritium and C14, as well as for the determination of H3/C14 dpm ratios in water-soluble biological samples by liquid scintillation counting techniques. A plot of H3/C14 cpm vs. H3/C14 dpm determined using the above system is linear over wider range than are two other counting systems developed for the counting of water-soluble samples. H3/C14 cpm ratios counted with the methyl Cellosolve-toluene system are relatively stable in the presence of HCl and NaCl contamination, and thus this solvent system can be used in the initial determinations of H3/C14 ratios of evaporated HCl hydrolyzates and other partially purified biological materials. Activities in dilute solutions of C14- and tritium-labeled proteins can also be differentially determined using this solvent system. 1963.

published proceedings

• Anal Biochem

author list (cited authors)

• PROCKOP, D. J., & EBERT, P. S.

citation count

• 91

complete list of authors

• PROCKOP, DJ||EBERT, PS

publication date

• January 1963

publisher

• Elsevier  Publisher

published in

• Analytical Biochemistry  Journal

keywords

• Biological Assay
• Carbon Isotopes
• Carbon Radioisotopes
• EXPERIMENTAL LAB STUDY
• Radiometry
• Research
• Tritium
• Water

Digital Object Identifier (DOI)

• 10.1016/0003-2697(63)90134-9

start page

• 263

end page

• 271

volume

• 6

issue

• 3

URL

• http://dx.doi.org/10.1016/0003-2697(63)90134-9