A simple method for differential assay of tritium and carbon-14 in water-soluble biological materials Academic Article uri icon


  • A methyl Cellosolve-toluene phosphor solvent system is described for the differential determination of absolute levels of tritium and C14, as well as for the determination of H3/C14 dpm ratios in water-soluble biological samples by liquid scintillation counting techniques. A plot of H3/C14 cpm vs. H3/C14 dpm determined using the above system is linear over wider range than are two other counting systems developed for the counting of water-soluble samples. H3/C14 cpm ratios counted with the methyl Cellosolve-toluene system are relatively stable in the presence of HCl and NaCl contamination, and thus this solvent system can be used in the initial determinations of H3/C14 ratios of evaporated HCl hydrolyzates and other partially purified biological materials. Activities in dilute solutions of C14- and tritium-labeled proteins can also be differentially determined using this solvent system. © 1963.

author list (cited authors)

  • Prockop, D. J., & Ebert, P. S.

citation count

  • 89

publication date

  • September 1963