Crystal induced inflammation in canine joints. 3. Evidence against bradykinin as a mediator of inflammation.
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abstract
Injection of urate crystals into canine joints induces an acute response associated with increase in intra-articular pressure, leukocyte accumulation, and a fall in pH. Single injections of bradykinin cause an abrupt, transient rise in pressure that can be greatly attenuated by prior injection of carboxypeptidase B. If bradykinin were an essential mediator of the inflammation induced by urate crystals, then the inflammatory response should be suppressed by carboxypeptidase B. This did not occur although joints treated with carboxypeptidase were unresponsive to injected bradykinin even at the peak of the inflammatory response to crystals; enzyme activity in the joint fluid exudate persisted throughout the reaction. These studies suggest that bradykinin is not a mediator of the acute inflammatory response induced by microcrystalline substances. 1966.