Quantitative assay for hydroxylysine in protein hydrolyzates
- Additional Document Info
- View All
A quantitative assay for hydroxylysine in protein hydrolyzates was developed on the basis of periodate oxidation of the hydroxyamino acid to glutamic semialdehyde and Δ1-pyrroline-5-carboxylic acid, and subsequent oxidation of these compounds to an unidentified product which formed a color with p-dimethylaminobenzaldehyde. Since proline gave the same color, a preliminary separation of hydroxylysine from proline was carried out by thin-layer chromatography. The assay will detect 2 μg of hydroxylysine. © 1971.
author list (cited authors)
Blumenkrantz, N., & Prockop, D. J.