Quantitative assay for hydroxylysine in protein hydrolyzates. Academic Article

abstract

• A quantitative assay for hydroxylysine in protein hydrolyzates was developed on the basis of periodate oxidation of the hydroxyamino acid to glutamic semialdehyde and 1-pyrroline-5-carboxylic acid, and subsequent oxidation of these compounds to an unidentified product which formed a color with p-dimethylaminobenzaldehyde. Since proline gave the same color, a preliminary separation of hydroxylysine from proline was carried out by thin-layer chromatography. The assay will detect 2 g of hydroxylysine. 1971.

authors

• Prockop, Darwin

published proceedings

• Anal Biochem

author list (cited authors)

• Blumenkrantz, N., & Prockop, D. J.

citation count

• 29

complete list of authors

• Blumenkrantz, N||Prockop, DJ

publication date

• January 1971

publisher

• Elsevier  Publisher

published in

• Analytical Biochemistry  Journal

keywords

• Aldehydes
• Amino Acids
• Chromatography, Thin Layer

Digital Object Identifier (DOI)

• 10.1016/0003-2697(71)90461-1

start page

• 59

end page

• 64

volume

• 39

issue

• 1

URL

• http://dx.doi.org/10.1016/0003-2697(71)90461-1