Quantitative assay for hydroxylysine in protein hydrolyzates. Academic Article uri icon


  • A quantitative assay for hydroxylysine in protein hydrolyzates was developed on the basis of periodate oxidation of the hydroxyamino acid to glutamic semialdehyde and 1-pyrroline-5-carboxylic acid, and subsequent oxidation of these compounds to an unidentified product which formed a color with p-dimethylaminobenzaldehyde. Since proline gave the same color, a preliminary separation of hydroxylysine from proline was carried out by thin-layer chromatography. The assay will detect 2 g of hydroxylysine. 1971.

published proceedings

  • Anal Biochem

author list (cited authors)

  • Blumenkrantz, N., & Prockop, D. J.

citation count

  • 29

complete list of authors

  • Blumenkrantz, N||Prockop, DJ

publication date

  • January 1971