Quantitative assay for hydroxylysine in protein hydrolyzates Academic Article uri icon

abstract

  • A quantitative assay for hydroxylysine in protein hydrolyzates was developed on the basis of periodate oxidation of the hydroxyamino acid to glutamic semialdehyde and Δ1-pyrroline-5-carboxylic acid, and subsequent oxidation of these compounds to an unidentified product which formed a color with p-dimethylaminobenzaldehyde. Since proline gave the same color, a preliminary separation of hydroxylysine from proline was carried out by thin-layer chromatography. The assay will detect 2 μg of hydroxylysine. © 1971.

author list (cited authors)

  • Blumenkrantz, N., & Prockop, D. J.

citation count

  • 29

publication date

  • January 1971