Labeled antibodies to protocollagen proline hydroxylase from chick embryos for intracellular localization of the enzyme. Academic Article

abstract

• Pure protocollagen proline hydroxylase was prepared from chick embryos with a new procedure involving affinity column chromatography. Rabbits were immunized with the enzyme and antisera from the rabbits were found to contain high titers of antibodies which were specific as tested by immunodiffusion and immunoelectrophoresis. The -globulin fraction from the antisera was labeled with peroxidase and the labeled antibodies were used to localize protocollagen proline hydroxylase in matrix-free tendon cells from chick embryos. Light microscopy indicated that the enzyme was distributed in localized regions of the cytoplasm. 1972.

authors

• Prockop, Darwin

published proceedings

• Biochim Biophys Acta

author list (cited authors)

• Berg, R. A., Olsen, B. R., & Prockop, D. J.

citation count

• 24

complete list of authors

• Berg, RA||Olsen, BR||Prockop, DJ

publication date

• January 1972

publisher

• Elsevier  Publisher

published in

• Biochimica et Biophysica Acta: international journal of biochemistry and biophysics  Journal

keywords

• Aldehydes
• Ammonium Sulfate
• Animals
• Carbon Isotopes
• Chemical Phenomena
• Chemical Precipitation
• Chemistry
• Chick Embryo
• Chromatography, Affinity
• Chromatography, Gel
• Collagen
• Cytoplasm
• Gamma-Globulins
• Histocytochemistry
• Immunodiffusion
• Immunoelectrophoresis
• Mixed Function Oxygenases
• Peroxidases
• Plants
• Procollagen-Proline Dioxygenase
• Rabbits
• Tendons

Digital Object Identifier (DOI)

• 10.1016/0005-2795(72)90188-2

start page

• 167

end page

• 175

volume

• 285

issue

• 1

URL

• http://dx.doi.org/10.1016/0005-2795(72)90188-2