Two improved methods for preparing ferritin-protein conjugates for electron microscopy. Academic Article uri icon


  • Two improved procedures were developed for activating ferritin so that the ferritin could be covalently linked to antibodies. One procedure involved use of a water-soluble carbodiimide and N-hydroxysuccinimide to prepare ferritin-containing activated esters. The other involved activation of the ferritin with excess glutaraldehyde. The ferritin-antibody conjugates prepared with the two procedures were shown to have a number of properties which made them suitable for locating antigenic components in cells.

published proceedings

  • J Cell Biol

author list (cited authors)

  • Kishida, Y., Olsen, B. R., Berg, R. A., & Prockop, D. J.

citation count

  • 157

complete list of authors

  • Kishida, Y||Olsen, BR||Berg, RA||Prockop, DJ

publication date

  • February 1975