Use of glutaraldehyde-fixed Escherichia coli cells as a convenient support for radioimmune assays. Academic Article

abstract

• Escherichia coli were activated by incubation with 1% glutaraldehyde, and the activated bacteria were then conjugated by incubation with IgG. About 66,000 molecules of IgG were bound per cell. The Escherichia coli-antibody conjugates were then used in competitive radioimmune assays. When a specific IgG fraction was used to prepare the conjugate, each cell bound about 16,000 molecules of antigen. With an antigen which had a specific activity of about 300 cpm/ng (or about 4.8 104 cpm/pmol), the assay was linear over a range of 40-300 ng/ml (0.3-2.0 pmol/ml). About 100 samples were assayed per day. 1977.

authors

• Prockop, Darwin

published proceedings

• Anal Biochem

author list (cited authors)

• Whei-Yang Kao, W., Guzman, N. A., & Prockop, D. J.

citation count

• 3

complete list of authors

• Whei-Yang Kao, W||Guzman, NA||Prockop, DJ

publication date

• January 1977

publisher

• Elsevier  Publisher

published in

• Analytical Biochemistry  Journal

keywords

• Animals
• Binding Sites, Antibody
• Escherichia Coli
• Glutaral
• Immunoglobulin G
• Indicators And Reagents
• Kinetics
• Rabbits
• Radioimmunoassay

Digital Object Identifier (DOI)

• 10.1016/0003-2697(77)90614-5

start page

• 209

end page

• 219

volume

• 81

issue

• 1

URL

• http://dx.doi.org/10.1016/0003-2697(77)90614-5