Use of glutaraldehyde-fixed Escherichia coli cells as a convenient support for radioimmune assays.
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Escherichia coli were activated by incubation with 1% glutaraldehyde, and the activated bacteria were then conjugated by incubation with IgG. About 66,000 molecules of IgG were bound per cell. The Escherichia coli-antibody conjugates were then used in competitive radioimmune assays. When a specific IgG fraction was used to prepare the conjugate, each cell bound about 16,000 molecules of antigen. With an antigen which had a specific activity of about 300 cpm/ng (or about 4.8 104 cpm/pmol), the assay was linear over a range of 40-300 ng/ml (0.3-2.0 pmol/ml). About 100 samples were assayed per day. 1977.
author list (cited authors)
Whei-Yang Kao, W., Guzman, N. A., & Prockop, D. J.
complete list of authors
Whei-Yang Kao, W||Guzman, NA||Prockop, DJ