Synthesis of an elastin component of molecular weight about 70,000 by polysomes from chick embryo aortas. Academic Article

abstract

• Polysomes were isolated from aortas of 17-day-old chick embryos, and the synthesis of the nascent polypeptide chains was completed in vitro. When a mixture of a labeled amino acids found in elastin was used, the major radioactive product obtained was of molecular weight about 70,000 and was similar to elastin by several criteria. The 70,000 molecular weight product was extractable in propanol-butanol, it was not labeled with [35S]methionine, and it was precipitated by antibodies against elastin. Polypeptides larger than 70,000 molecular weight were also synthesized but these larger polypeptides incorporated relatively small amounts of [14C]valine, and they appeared to represent pro chains of procollagen. The results suggest that the major gene product for elastin has a molecular weight of about 70,000. 1978.

authors

• Prockop, Darwin

published proceedings

• Arch Biochem Biophys

author list (cited authors)

• Ryhnen, L., Graves, P. N., Bressan, G. M., & Prockop, D. J.

citation count

• 22

complete list of authors

• Ryhänen, L||Graves, PN||Bressan, GM||Prockop, DJ

publication date

• January 1978

publisher

• Elsevier  Publisher

published in

• Archives of Biochemistry and Biophysics  Journal

keywords

• Amino Acids
• Animals
• Aorta
• Carcinoma, Ehrlich Tumor
• Chick Embryo
• Elastin
• Molecular Weight
• Polyribosomes
• Reticulocytes

Digital Object Identifier (DOI)

• 10.1016/0003-9861(78)90176-5

start page

• 344

end page

• 351

volume

• 185

issue

• 2

URL

• http://dx.doi.org/10.1016/0003-9861(78)90176-5