Time lag in the secretion of collagen by matrix-free tendon cells and inhibition of the secretory process by colchicine and vinblastine. Academic Article uri icon

abstract

  • Matrix-free cells were isolated from chick embryo tendons and incubated with [14Cproline in order to follow the time course for the synthesis and secretion of [14C]-collagen. The results indicated a lag of 2-3 min between the time at which the incorporation of [14C]proline and the synthesis of peptide-bound [14C]hydroxyproline reached steady-state levels. A lag of about 18 min was observed between the initiation of the synthesis of collagen [14C]hydroxyproline and the secretion of [14C]collagen from the cells into the incubation medium. Colchicine and vinblastine were found to inhibit the rate of secretion of [14C]-collagen by about 70% and with appropriate concentrations of these agents the amount of intracellular [14C]collagen increased about 2-fold in 2 h. The results suggested that the secretion of collagen depends at least in part on the intensity of microtubules. Addition of dibutyryl cyclic AMP to the incubation medium produced a small increase in the fraction of total [14C]collagen which was secreted into the medium by the cells in 1 h. 1972.

published proceedings

  • Biochim Biophys Acta

author list (cited authors)

  • Dehm, P., & Prockop, D. J.

citation count

  • 199

complete list of authors

  • Dehm, P||Prockop, DJ

publication date

  • April 1972