Can proline analogues be used to prevent fibroblasts from overgrowing cultures of epithelial cells? Academic Article

abstract

• One of the problems in culturing epithelial cells is that cultures of such cells are frequently overgrown by fibroblasts. We have recently made an observation which apparently provides a new technique for eliminating fibroblasts from cultures of mixed cell populations. We have tested this technique with 2 different kinds of fibroblasts and with 2 nonfibroblastic cell lines. It has also been tested with a few additional cell lines by other investigators. We have not, however, tested the procedure with epithelial cells and therefore we present the technique here only as a proposal which may be useful to investigators who are actively working with cultures of epithelial cells.

published proceedings

• Birth Defects Orig Artic Ser

author list (cited authors)

• Kao, W. W., & Prockop, D. J.

citation count

• 3

complete list of authors

• Kao, WW||Prockop, DJ

publication date

• December 1980

published in

• Birth defects original article series  Journal

keywords

• Animals
• Chick Embryo
• Collagen
• Culture Techniques
• Depression, Chemical
• Dose-Response Relationship, Drug
• Epithelial Cells
• Fibroblasts
• Humans
• Hydroxyproline

PubMed Central ID

• 7213980

start page

• 53

end page

• 59

volume

• 16

issue

• 2