Assessment of small polymorphisms in defined human collagen gene segments. Academic Article

abstract

• Restriction fragment length polymorphisms (RFLPs) greater than 1.5 kb in size have been identified in all but one of the human fibrillar collagen genes. However, the number of informative RFLPs for these genes is still limited. Here we present the conjunct use of two techniques for the assessment of small length variations within defined segments of the genome. This strategy has the potential to be used for the allelic exclusion of cloned genomic fragments prior to sequencing. Moreover it can be a useful and sensitive tool to determine if genetic linkage exists between abnormal phenotypes and loci where conventional Southern blotting analysis has failed to detect informative RFLPs.

authors

• Prockop, Darwin

published proceedings

• Hum Genet

author list (cited authors)

• Sippola-Thiele, M., Tromp, G. C., Prockop, D. J., & Ramirez, F.

citation count

• 0

complete list of authors

• Sippola-Thiele, M||Tromp, GC||Prockop, DJ||Ramirez, F

publication date

• March 1986

publisher

• Springer Nature  Publisher

published in

• Human Genetics  Journal

keywords

• Chromosome Mapping
• Collagen
• DNA
• DNA Restriction Enzymes
• Genes
• Humans
• Polymorphism, Genetic

Digital Object Identifier (DOI)

• 10.1007/BF00291887

start page

• 245

end page

• 247

volume

• 72

issue

• 3

URL

• http://dx.doi.org/10.1007/bf00291887