Collagen synthesis: localization of prolyl hydroxylase in tendon cells detected with ferritin-labeled antibodies. uri icon

abstract

  • An improved procedure was employed for linking ferritin to antibodies against prolyl hydroxylase, the enzyme that synthesizes the hydroxyproline in collagen. By electron microscopy, the enzyme was then found to be localized in cisternae of the rough endoplasmic reticulum of embryonic tendon cells; this indicates that hydroxylation of proline occurs while newly synthesized polypeptides are fed into the cisternae.

published proceedings

  • Science

author list (cited authors)

  • Olsen, B. R., Berg, R. A., Kishida, Y., & Prockop, D. J.

citation count

  • 104

complete list of authors

  • Olsen, BR||Berg, RA||Kishida, Y||Prockop, DJ

publication date

  • November 1973