Collagen synthesis: localization of prolyl hydroxylase in tendon cells detected with ferritin-labeled antibodies. Academic Article

abstract

• An improved procedure was employed for linking ferritin to antibodies against prolyl hydroxylase, the enzyme that synthesizes the hydroxyproline in collagen. By electron microscopy, the enzyme was then found to be localized in cisternae of the rough endoplasmic reticulum of embryonic tendon cells; this indicates that hydroxylation of proline occurs while newly synthesized polypeptides are fed into the cisternae.

authors

• Prockop, Darwin

published proceedings

• Science

author list (cited authors)

• Olsen, B. R., Berg, R. A., Kishida, Y., & Prockop, D. J.

citation count

• 104

complete list of authors

• Olsen, BR||Berg, RA||Kishida, Y||Prockop, DJ

publication date

• November 1973

publisher

• American Association for the Advancement of Science (AAAS)  Publisher

published in

• Science  Journal

keywords

• Animals
• Chick Embryo
• Endoplasmic Reticulum
• Ferritins
• Hemagglutination Inhibition Tests
• Hydroxyproline
• Immunoassay
• Methods
• Microscopy, Electron
• Procollagen-Proline Dioxygenase
• Rabbits
• Tendons

PubMed Central ID

• 4355681

Digital Object Identifier (DOI)

• 10.1126/science.182.4114.825

start page

• 825

end page

• 827

volume

• 182

issue

• 4114

URL

• http://dx.doi.org/10.1126/science.182.4114.825