Separation of human pro alpha 1 (I) and pro alpha 2 (I) procollagen chains by reverse phase high performance liquid chromatography. Academic Article uri icon

abstract

  • Human pro alpha 1 (I) and pro alpha 2 (I) procollagen chains have been separated on a large por (33 nm) reverse phase high performance liquid chromatography C18 column using an acetonitrile aqueous gradient containing 9 mM trifluoroacetic acid. The separation of the chains was evaluated by gel electrophoresis. The yield of the chromatography, based on radiolabel recovery, is equal to or better than 80%. Absorbance monitoring at 210 nm permits detection of less than 5 micrograms procollagen.

published proceedings

  • Coll Relat Res

author list (cited authors)

  • Van der Rest, M., Stolle, C. A., Prockop, D. J., & Fietzek, P. P.

citation count

  • 3

complete list of authors

  • Van der Rest, M||Stolle, CA||Prockop, DJ||Fietzek, PP

publication date

  • January 1982