Ferritin-conjugated antibodies used for labeling of organelles involved in the cellular synthesis and transport of procollagen. Academic Article

abstract

• An improved procedure was used to conjugate ferritin to antibodies specific for the NH(2)-terminal extensions on the precursor form of collagen known as procollagen. The ferritin-antibody conjugates were then used to determine the localization of procollagen in fibroblasts isolated from chick-embryo tendons. Procollagen was found in the cisternae of the endoplasmic reticulum, indicating that the protein passes into this compartment early in its biosynthesis. Specific labeling of large Golgi vacuoles was also observed, suggesting that this compartment is also involved in the secretory process. When cells were incubated with colchicine so that the secretion of procollagen was delayed, there was an increase of large, smooth-surfaced vacuoles in the cells and these vacuoles were labeled with the ferritin-antibody conjugate.

authors

• Prockop, Darwin

published proceedings

• Proc Natl Acad Sci U S A

altmetric score

• 3

author list (cited authors)

• Olsen, B. R., & Prockop, D. J.

citation count

• 81

complete list of authors

• Olsen, BR||Prockop, DJ

publication date

• May 1974

publisher

• Proceedings of the National Academy of Sciences  Publisher

published in

• Proceedings of the National Academy of Sciences of the United States of America  Journal

keywords

• Animals
• Antibodies
• Chick Embryo
• Collagen
• Endoplasmic Reticulum
• Ferritins
• Fibroblasts
• Golgi Apparatus
• Histocytochemistry
• Methods
• Microscopy, Electron
• Protein Precursors
• Rabbits

PubMed Central ID

• 4525314

Digital Object Identifier (DOI)

• 10.1073/pnas.71.5.2033

start page

• 2033

end page

• 2037

volume

• 71

issue

• 5

URL

• http://dx.doi.org/10.1073/pnas.71.5.2033