Enhanced in situ detection of beta-glucuronidase activity in murine tissue. Academic Article

abstract

• We outline here a protocol for high-resolution in situ localization of beta-glucuronidase in murine tissues processed in glycol methacrylate (GMA). Murine tissues were first stained with 5-bromo-4-chloro-3-indolyl-beta-d-glucuronic acid (x-gluc), followed by histological processing in GMA. Retention of the blue indigo reaction product after overnight incubations in x-gluc allowed high-resolution localization of beta-glucuronidase activity by brightfield microscopy. When illuminated under darkfield, the x-gluc signal was enhanced, permitting detection even in cells with low-level enzyme activity. This technique offers for the first time a more sensitive enzyme histochemical method of detecting beta-glucuronidase activity in animal tissues and also the opportunity to examine expression at high magni-fication.

authors

• Prockop, Darwin

published proceedings

• J Histochem Cytochem

author list (cited authors)

• Kopen, G. C., Prockop, D. J., & Phinney, D. G.

citation count

• 4

complete list of authors

• Kopen, GC||Prockop, DJ||Phinney, DG

publication date

• July 1999

publisher

• SAGE Publications  Publisher

published in

• Journal of Histochemistry and Cytochemistry: imaging the spectrum of cell biology  Journal

keywords

• Animals
• Glucuronates
• Glucuronic Acid
• Glucuronidase
• Immunoenzyme Techniques
• Liver
• Methacrylates
• Mice
• Spleen

PubMed Central ID

• 10375385

Digital Object Identifier (DOI)

• 10.1177/002215549904700714

start page

• 965

end page

• 968

volume

• 47

issue

• 7

URL

• http://dx.doi.org/10.1177/002215549904700714