In vitro differentiation of human marrow stromal cells into early progenitors of neural cells by conditions that increase intracellular cyclic AMP. Academic Article

abstract

• Human marrow stromal cells (hMSCs) are multipotential stem cells that can be differentiated into bone, cartilage, fat, and muscle. In the experiments here, we found that undifferentiated cultures of hMSCs express some markers characteristic of neural cells such as microtubule-associated protein 1B (MAP1B), neuron-specific tubulin (TuJ-1), neuron-specific enolase (NSE), and vimentin. By treating hMSCs with 0.5 mM isobutylmethylxanthine (IBMX)/1 mM dibutyryl cyclic AMP (dbcAMP) for 6 days, about 25% of the hMSCs differentiated into cells with a typical neural cell morphology and with increased levels of both NSE and vimentin. The data suggested that the hMSCs may have been differentiated into early progenitors of neural cells in vitro under conditions that increase the intracellular level of cAMP.

authors

• Prockop, Darwin

published proceedings

• Biochem Biophys Res Commun

altmetric score

• 6

author list (cited authors)

• Deng, W., Obrocka, M., Fischer, I., & Prockop, D. J.

citation count

• 403

complete list of authors

• Deng, W||Obrocka, M||Fischer, I||Prockop, DJ

publication date

• January 2001

publisher

• Elsevier  Publisher

published in

• Biochemical and Biophysical Research Communications  Journal

keywords

• 1-Methyl-3-isobutylxanthine
• Adolescent
• Adult
• Bone Marrow Cells
• Bucladesine
• Cell Differentiation
• Cells, Cultured
• Cyclic AMP
• Humans
• Microtubule-Associated Proteins
• Middle Aged
• Nervous System
• Phenotype
• Phosphopyruvate Hydratase
• Stromal Cells
• Tubulin
• Vimentin

Digital Object Identifier (DOI)

• 10.1006/bbrc.2001.4570

start page

• 148

end page

• 152

volume

• 282

issue

• 1

URL

• http://dx.doi.org/10.1006/bbrc.2001.4570