Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells. Academic Article

abstract

• Marrow stromal cells are adult stem cells from bone marrow that can differentiate into multiple nonhematopoietic cell lineages. Previous reports demonstrated that single-cell-derived colonies of marrow stromal cells contained two morphologically distinct cell types: spindle-shaped cells and large flat cells. Here we found that early colonies also contain a third kind of cell: very small round cells that rapidly self-renew. Samples enriched for the small cells had a greater potential for multipotential differentiation than samples enriched for the large cells. Also, the small cells expressed a series of surface epitopes and other proteins that potentially can be used to distinguish the small cells from the large cells. The results suggested it will be important to distinguish the major subpopulations of marrow stromal cells in defining their biology and their potential for cell and gene therapy.

authors

• Prockop, Darwin

published proceedings

• Proc Natl Acad Sci U S A

altmetric score

• 9

author list (cited authors)

• Colter, D. C., Sekiya, I., & Prockop, D. J.

citation count

• 736

complete list of authors

• Colter, DC||Sekiya, I||Prockop, DJ

publication date

• July 2001

publisher

• Proceedings of the National Academy of Sciences  Publisher

published in

• Proceedings of the National Academy of Sciences of the United States of America  Journal

keywords

• Adult
• Cell Differentiation
• Cell Division
• Cell Lineage
• Humans
• Stem Cells
• Stromal Cells

PubMed Central ID

• 11427725

Digital Object Identifier (DOI)

• 10.1073/pnas.141221698

start page

• 7841

end page

• 7845

volume

• 98

issue

• 14

URL

• http://dx.doi.org/10.1073/pnas.141221698