Characterization of 2 aquareovirus proteins. Academic Article uri icon

abstract

  • The protein products of aquareovirus genome segments 10 and 11 were investigated. The genome segment 10 encodes the major outer capsid protein (VP7) of aquareovirus. The VP7 protein has a molecular weight of 35 kDa and was found to be glycosylated. The electrophoretic mobility of VP7 in SDS-PAGE was unaltered when untreated and deglycosylated proteins were compared, indicating the presence of short oligosaccharide chains. Selective binding of lectins with well-defined sugar specificities suggest that most of the short N-linked carbohydrate chains of VP7 contain galactose residues. Genome segment 11 encoded 2 non-structural proteins (NS29 and NS15). Partial protease mapping indicated that the NS29 and NS15 proteins are derived from initiation of protein synthesis at 2 distinct sites.

author list (cited authors)

  • Samal, S. K., Subramanian, K., & Lupiani, B.

citation count

  • 2

publication date

  • January 1995