Characterization of 2 aquareovirus proteins. Academic Article

abstract

• The protein products of aquareovirus genome segments 10 and 11 were investigated. The genome segment 10 encodes the major outer capsid protein (VP7) of aquareovirus. The VP7 protein has a molecular weight of 35 kDa and was found to be glycosylated. The electrophoretic mobility of VP7 in SDS-PAGE was unaltered when untreated and deglycosylated proteins were compared, indicating the presence of short oligosaccharide chains. Selective binding of lectins with well-defined sugar specificities suggest that most of the short N-linked carbohydrate chains of VP7 contain galactose residues. Genome segment 11 encoded 2 non-structural proteins (NS29 and NS15). Partial protease mapping indicated that the NS29 and NS15 proteins are derived from initiation of protein synthesis at 2 distinct sites.

authors

• Lupiani, Blanca

published proceedings

• Vet Res

author list (cited authors)

• Samal, S. K., Subramanian, K., & Lupiani, B.

citation count

• 2

complete list of authors

• Samal, SK||Subramanian, K||Lupiani, B

publication date

• December 1995

keywords

• Animals
• Antigens, Viral
• Bass
• Capsid
• Capsid Proteins
• Cell Line
• Embryo, Nonmammalian
• Genome, Viral
• Glycosylation
• Protein Biosynthesis
• Protein Processing, Post-Translational
• RNA, Double-Stranded
• RNA, Viral
• Reoviridae
• Salmon

start page

• 388

end page

• 393

volume

• 26

issue

• 5-6