Avian influenza viral nucleocapsid and hemagglutinin proteins induce chicken CD8+ memory T lymphocytes. Academic Article

abstract

• The avian influenza viruses (AIVs) can be highly contagious to poultry and a zoonotic threat to humans. Since the memory CD8(+) T lymphocyte responses in chickens to AIV proteins have not been defined, these responses to H5N9 AIV hemagglutinin (HA) and nucleocapsid (NP) proteins were evaluated by ex vivo stimulation with virus infected non-professional antigen presenting cells. Secretion of IFNgamma by activated T lymphocytes was evaluated through macrophage induction of nitric oxide. AIV specific, MHC-I restricted memory CD8(+) T lymphocyte responses to NP and HA were observed 3 to 9 weeks post-inoculation (p.i.). The responses specific to NP were greater than those to HA with maximum responses being observed at 5 weeks p.i. followed by a decline to weakly detectable levels by 9 weeks p.i. The cross-reaction of T lymphocytes to a heterologous H7N2 AIV strain demonstrated their ability to respond to a broader range of AIV.

authors

• Lupiani, Blanca

published proceedings

• Virology

altmetric score

• 6

author list (cited authors)

• Singh, S., Briles, W. E., Lupiani, B., & Collisson, E. W.

citation count

• 16

complete list of authors

• Singh, Shailbala||Briles, Worthie E||Lupiani, Blanca||Collisson, Ellen W

publication date

• April 2010

publisher

• Elsevier  Publisher

published in

• Virology  Journal

keywords

• Animals
• Antibodies, Viral
• CD8-Positive T-Lymphocytes
• Cells, Cultured
• Chickens
• Cross Reactions
• Hemagglutination Inhibition Tests
• Hemagglutinin Glycoproteins, Influenza Virus
• Immunologic Memory
• Influenza A Virus
• Influenza In Birds
• Interferon-gamma
• Macrophages
• Nitric Oxide
• Nucleocapsid Proteins

PubMed Central ID

• 20116819

Digital Object Identifier (DOI)

• 10.1016/j.virol.2009.12.029

start page

• 231

end page

• 238

volume

• 399

issue

• 2

URL

• http://dx.doi.org/10.1016/j.virol.2009.12.029