Detection and characterization of a distinct bornavirus lineage from healthy Canada geese (Branta canadensis). Academic Article

abstract

• Avian bornaviruses (ABV), identified in 2008, infect captive parrots and macaws worldwide. The natural reservoirs of these viruses are unknown. Reverse transcription-PCR (RT-PCR) was used to screen oropharyngeal/cloacal swab and brain samples from wild Canada geese (Branta canadensis) for ABV. Approximately 2.9% of swab samples were positive for bornavirus sequences. Fifty-two percent of brain samples from 2 urban flocks also tested positive, and brain isolates were cultured in duck embryo fibroblasts. Phylogenetic analyses placed goose isolates in an independent cluster, and more notably, important regulatory sequences present in Borna disease virus but lacking in psittacine ABVs were present in goose isolates.

authors

• Ferro, Pamela
• Heatley, J.
• Lupiani, Blanca
• Payne, Susan
• Tizard, Ian

published proceedings

• J Virol

author list (cited authors)

• Payne, S., Covaleda, L., Jianhua, G., Swafford, S., Baroch, J., Ferro, P. J., ... Tizard, I.

citation count

• 63

complete list of authors

• Payne, Susan||Covaleda, Lina||Jianhua, Guo||Swafford, Seth||Baroch, John||Ferro, Pamela J||Lupiani, Blanca||Heatley, Jill||Tizard, Ian

publication date

• November 2011

publisher

• American Society for Microbiology  Publisher

published in

• Journal of Virology  Journal

keywords

• Animals
• Anseriformes
• Bornaviridae
• Brain
• Canada
• Cell Line
• Cloaca
• Cluster Analysis
• Molecular Sequence Data
• Oropharynx
• Phylogeny
• RNA, Viral
• Reverse Transcriptase Polymerase Chain Reaction
• Sequence Analysis, DNA

PubMed Central ID

• 21900161

Digital Object Identifier (DOI)

• 10.1128/JVI.05700-11

URI

• https://hdl.handle.net/1969.1/182394

start page

• 12053

end page

• 12056

volume

• 85

issue

• 22

URL

• http://dx.doi.org/10.1128/jvi.05700-11