Construction of strains for rapid homokaryon purification after integration of constructs at the histidine-3 ( his-3) locus of Neurospora crassa. Academic Article uri icon


  • We report the construction of histidine-3 (his-3) strains of Neurospora crassa containing the hygromycin B phosphotransferase gene of Escherichia coli (hph(+)) fused in-frame to the herpes simplex virus thymidine kinase gene (tk(+); Lupton et al. 1991), integrated at the his-3 locus. We also report the construction of two ampicillin-resistant and two kanamycin-resistant his-3 gene-replacement vector plasmids. The combined use of these strains and plasmids for his-3-targeted gene integration allows for the rapid identification of homokaryotic transformants containing the expected gene replacement event.

published proceedings

  • Curr Genet

author list (cited authors)

  • Lee, D. W., Haag, J. R., & Aramayo, R.

citation count

  • 7

complete list of authors

  • Lee, Dong W||Haag, Jeremy R||Aramayo, Rodolfo

publication date

  • April 2003