Construction of strains for rapid homokaryon purification after integration of constructs at the histidine-3 ( his-3) locus of Neurospora crassa. Academic Article

abstract

• We report the construction of histidine-3 (his-3) strains of Neurospora crassa containing the hygromycin B phosphotransferase gene of Escherichia coli (hph(+)) fused in-frame to the herpes simplex virus thymidine kinase gene (tk(+); Lupton et al. 1991), integrated at the his-3 locus. We also report the construction of two ampicillin-resistant and two kanamycin-resistant his-3 gene-replacement vector plasmids. The combined use of these strains and plasmids for his-3-targeted gene integration allows for the rapid identification of homokaryotic transformants containing the expected gene replacement event.

authors

• Aramayo, Rodolfo

published proceedings

• Curr Genet

author list (cited authors)

• Lee, D. W., Haag, J. R., & Aramayo, R.

citation count

• 7

complete list of authors

• Lee, Dong W||Haag, Jeremy R||Aramayo, Rodolfo

publication date

• April 2003

publisher

• Springer Nature  Publisher

published in

• Current Genetics  Journal

keywords

• Gene Transfer Techniques
• Histidine
• Neurospora Crassa
• Phosphotransferases (Alcohol Group Acceptor)

PubMed Central ID

• 12684841

Digital Object Identifier (DOI)

• 10.1007/s00294-002-0366-z

start page

• 17

end page

• 23

volume

• 43

issue

• 1

URL

• http://dx.doi.org/10.1007/s00294-002-0366-z