Hyperpolarized binding pocket nuclear Overhauser effect for determination of competitive ligand binding. Academic Article uri icon

abstract

  • Protein-mediated polarization transfer: Ligands L1 and L2 that competitively bind to a protein are subject to indirect spin-polarization transfer through the binding site of the protein. If protons H L1 of one ligand are hyperpolarized by dynamic nuclear polarization (DNP, see picture), signal intensities in the NMR spectrum of the second ligand become enhanced. The relative build-up of signal of the second ligand yields information on its binding epitope. Copyright 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

published proceedings

  • Angew Chem Int Ed Engl

altmetric score

  • 9.37

author list (cited authors)

  • Lee, Y., Zeng, H., Mazur, A., Wegstroth, M., Carlomagno, T., Reese, M., ... Hilty, C.

citation count

  • 31

publication date

  • January 2012

publisher