Isolation of cardiac myocytes and fibroblasts from neonatal rat pups. Chapter

abstract

• Neonatal rat ventricular myocytes (NRVM) and fibroblasts (FBs) serve as in vitro models for studying fundamental mechanisms underlying cardiac pathologies, as well as identifying potential therapeutic targets. Both cell types are relatively easy to culture as monolayers and can be manipulated using molecular and pharmacological tools. Because NRVM cease to proliferate after birth, and FBs undergo phenotypic changes and senescence after a few passages in tissue culture, primary cultures of both cell types are required for experiments. Below we describe methods that provide good cell yield and viability of primary cultures of NRVM and FBs from 0 to 3-day-old neonatal rat pups.

author list (cited authors)

• Golden, H. B., Gollapudi, D., Gerilechaogetu, F., Li, J., Cristales, R. J., Peng, X. u., & Dostal, D. E.

citation count

• 64

complete list of authors

• Golden, Honey B||Gollapudi, Deepika||Gerilechaogetu, Fnu||Li, Jieli||Cristales, Ricardo J||Peng, Xu||Dostal, David E

editor list (cited editors)

• Peng, X., & Antonyak, M.

editors

• Peng, Xu

Book Title

• CARDIOVASCULAR DEVELOPMENT: METHODS AND PROTOCOLS

publication date

• February 2012

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Animals
• Animals, Newborn
• Cell Adhesion
• Cell Separation
• Cell Survival
• Fibroblasts
• Heart Ventricles
• Myocytes, Cardiac
• Rats
• Tissue And Organ Harvesting

PubMed Central ID

• 22222535

Digital Object Identifier (DOI)

• 10.1007/978-1-61779-523-7_20

International Standard Book Number (ISBN) 13

• 978-1-61779-522-0

start page

• 205

end page

• 214

volume

• 843

URL

• http://dx.doi.org/10.1007/978-1-61779-523-7_20

user-defined tag

• 3 Good Health and Well-Being