STEM FOOTPRINTS AND BOUND MASS DISTRIBUTIONS FOR DNA CONTROL PROTEINS

abstract

• We review a current methodology which analyzes quantitative scattering data from Scanning Transmission Electron Microscopy to establish footprintspositions of DNA-binding proteins on cloned genesand mass distributions for the bound protein. Recognition by computer of the DNA path and the extent of the bound protein leads to an analysis rate which is high for image-based data. The methods have been applied in the study of eukaryotic transcription initiation and T antigen interaction with the SV40 control region. 1985, Nature Publishing Group. All rights reserved.

authors

• Wilson, Van

published proceedings

• BIO-TECHNOLOGY

author list (cited authors)

• HOUGH, P., MASTRANGELO, I. A., WALL, J. S., HAINFELD, J. F., WILSON, V. G., RYDER, K., & TEGTMEYER, P.

citation count

• 2

complete list of authors

• HOUGH, PVC||MASTRANGELO, IA||WALL, JS||HAINFELD, JF||WILSON, VG||RYDER, K||TEGTMEYER, P

publication date

• January 1985

publisher

• Springer Nature  Publisher

published in

• Bio/technology (Nature Publishing Company)  Journal

keywords

• Generic Health Relevance
• Genetics

Digital Object Identifier (DOI)

• 10.1038/nbt0685-549

start page

• 549

end page

• 553

volume

• 3

issue

• 6

URL

• http://dx.doi.org/10.1038/nbt0685-549