A genetic analysis of glucocorticoid receptor signaling. Identification and characterization of ligand-effect modulators in Saccharomyces cerevisiae.

abstract

• To find novel components in the glucocorticoid signal transduction pathway, we performed a yeast genetic screen to identify ligand-effect modulators (LEMs), proteins that modulate the cellular response to hormone. We isolated several mutants that conferred increased glucocorticoid receptor (GR) activity in response to dexamethasone and analyzed two of them in detail. These studies identify two genes, LEM3 and LEM4, which correspond to YNL323w and ERG6, respectively. LEM3 is a putative transmembrane protein of unknown function, and ERG6 is a methyltransferase in the ergosterol biosynthetic pathway. Analysis of null mutants indicates that LEM3 and ERG6 act at different steps in the GR signal transduction pathway.

authors

• Sitcheran, Raquel

published proceedings

• Genetics

author list (cited authors)

• Sitcheran, R., Emter, R., Kralli, A., & Yamamoto, K. R.

citation count

• 25

complete list of authors

• Sitcheran, R||Emter, R||Kralli, A||Yamamoto, KR

publication date

• November 2000

publisher

• Oxford University Press (OUP)  Publisher

published in

• Genetics  Journal

keywords

• ATP-Binding Cassette Transporters
• Animals
• Base Sequence
• Canavanine
• Cloning, Molecular
• Crosses, Genetic
• DNA Primers
• Dexamethasone
• Fungal Proteins
• Genetic Complementation Test
• Membrane Proteins
• Methyltransferases
• Molecular Sequence Data
• Mutagenesis
• Polymerase Chain Reaction
• Receptors, Glucocorticoid
• Recombinant Proteins
• Saccharomyces Cerevisiae
• Saccharomyces Cerevisiae Proteins
• Signal Transduction
• Ultraviolet Rays

PubMed Central ID

• 11063677

Digital Object Identifier (DOI)

• 10.1093/genetics/156.3.963

start page

• 963

end page

• 972

volume

• 156

issue

• 3

URL

• http://dx.doi.org/10.1093/genetics/156.3.963