PCM-1, A 228-kD centrosome autoantigen with a distinct cell cycle distribution. Academic Article uri icon


  • We report the identification and primary sequence of PCM-1, a 228-kD centrosomal protein that exhibits a distinct cell cycle-dependent association with the centrosome complex. Immunofluorescence microscopy using antibodies against recombinant PCM-1 demonstrated that PCM-1 is tightly associated with the centrosome complex through G1, S, and a portion of G2. However, late in G2, as cells prepare for mitosis, PCM-1 dissociates from the centrosome and then remains dispersed throughout the cell during mitosis before re-associating with the centrosomes in the G1 phase progeny cells. These results demonstrate that the pericentriolar material is a dynamic substance whose composition can fluctuate during the cell cycle.

published proceedings

  • J Cell Biol

altmetric score

  • 3

author list (cited authors)

  • Balczon, R., Bao, L., & Zimmer, W. E.

citation count

  • 122

complete list of authors

  • Balczon, R||Bao, L||Zimmer, WE

publication date

  • March 1994