PCM-1, A 228-kD centrosome autoantigen with a distinct cell cycle distribution. Academic Article

abstract

• We report the identification and primary sequence of PCM-1, a 228-kD centrosomal protein that exhibits a distinct cell cycle-dependent association with the centrosome complex. Immunofluorescence microscopy using antibodies against recombinant PCM-1 demonstrated that PCM-1 is tightly associated with the centrosome complex through G1, S, and a portion of G2. However, late in G2, as cells prepare for mitosis, PCM-1 dissociates from the centrosome and then remains dispersed throughout the cell during mitosis before re-associating with the centrosomes in the G1 phase progeny cells. These results demonstrate that the pericentriolar material is a dynamic substance whose composition can fluctuate during the cell cycle.

authors

• Zimmer, Warren

published proceedings

• J Cell Biol

altmetric score

• 3

author list (cited authors)

• Balczon, R., Bao, L., & Zimmer, W. E.

citation count

• 122

complete list of authors

• Balczon, R||Bao, L||Zimmer, WE

publication date

• March 1994

publisher

• Rockefeller University Press  Publisher

published in

• The Journal of Cell Biology  Journal

keywords

• Amino Acid Sequence
• Animals
• Autoantigens
• Base Sequence
• CHO Cells
• Cell Cycle
• Cell Cycle Proteins
• Cricetinae
• DNA, Complementary
• Electrophoresis, Polyacrylamide Gel
• Fetus
• Fluorescent Antibody Technique
• Hela Cells
• Humans
• Immunoblotting
• Liver
• Molecular Sequence Data
• Organelles

PubMed Central ID

• 8120099

Digital Object Identifier (DOI)

• 10.1083/jcb.124.5.783

start page

• 783

end page

• 793

volume

• 124

issue

• 5

URL

• http://dx.doi.org/10.1083/jcb.124.5.783