Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.

abstract

• The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.

authors

• VanBuren, Vincent

published proceedings

• Genome Biol

author list (cited authors)

• Carter, M. G., Sharov, A. A., VanBuren, V., Dudekula, D. B., Carmack, C. E., Nelson, C., & Ko, M.

citation count

• 103

publication date

• January 2005

publisher

• Springer Nature  Publisher

keywords

• Animals
• Base Sequence
• Expressed Sequence Tags
• Gene Expression Profiling
• Genome
• Introns
• Mice
• Models, Genetic
• Oligonucleotide Array Sequence Analysis
• Oligonucleotide Probes
• Open Reading Frames
• RNA, Messenger
• Restriction Mapping
• Reverse Transcriptase Polymerase Chain Reaction
• Saccharomyces Cerevisiae
• Transcription, Genetic

PubMed Central ID

• 15998450

Digital Object Identifier (DOI)

• 10.1186/gb-2005-6-7-r61

start page

• R61

end page

• R61

volume

• 6

issue

• 7

URL

• http://dx.doi.org/10.1186/gb-2005-6-7-r61