Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray Academic Article uri icon

abstract

  • The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.

author list (cited authors)

  • Carter, M. G., Sharov, A. A., VanBuren, V., Dudekula, D. B., Carmack, C. E., Nelson, C., & Ko, M. S.

citation count

  • 100

publication date

  • July 2005