Assembly, Verification, and Initial Annotation of the NIA Mouse 7.4K cDNA Clone Set Academic Article uri icon

abstract

  • A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ~120,000 cDNA clones, which were initially re-arrayed into a set of ~11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.

altmetric score

  • 6

author list (cited authors)

  • VanBuren, V., Piao, Y., Dudekula, D. B., Qian, Y., Carter, M. G., Martin, P. R., ... Ko, M.

citation count

  • 45

publication date

  • December 2002