Cloning and analysis of chicken acetylcholinesterase transcripts from muscle and brain. Academic Article

abstract

• We have isolated cDNA clones from an embryonic chicken muscle cDNA library which encodes the complete catalytic T subunit of acetylcholinesterase. The deduced polypeptide comprises 767 amino acids, shows approximately 60% homology to acetylcholinesterases from other vertebrates and contains a 155 amino acid sequence inserted into the middle of the peptide which is unique to the chicken enzyme. Northern blots of embryonic chicken muscle and adult brain show three transcripts approximately 4.5, 5.5, and 6.0 kb hybridizing to a cDNA fragment of AChE. The 6.0 kb transcript is expressed transiently in embryonic muscle and is a major transcript in adult brain.

authors

• Rimer, Mendell

published proceedings

• Biochim Biophys Acta

author list (cited authors)

• Randall, W. R., Rimer, M., & Gough, N. R.

citation count

• 21

complete list of authors

• Randall, WR||Rimer, M||Gough, NR

publication date

• January 1994

publisher

• Elsevier  Publisher

published in

• Biochimica et Biophysica Acta: international journal of biochemistry and biophysics  Journal

keywords

• ACETYLCHOLINESTERASE
• Amino Acid Sequence
• Animals
• Base Sequence
• Brain
• Chickens
• Cloning, Molecular
• Isoenzymes
• Mice
• Molecular Sequence Data
• Muscles
• Protein Structure, Secondary
• Recombinant Proteins
• Restriction Mapping
• Sequence Deletion
• Sequence Homology, Amino Acid
• Torpedo
• Transcription, Genetic
• Vertebrates

Digital Object Identifier (DOI)

• 10.1016/0167-4781(94)90204-6

start page

• 453

end page

• 456

volume

• 1218

issue

• 3

URL

• http://dx.doi.org/10.1016/0167-4781(94)90204-6