Genetic interaction mapping reveals a role for the SWI/SNF nucleosome remodeler in spliceosome activation in fission yeast. Academic Article uri icon


  • Although numerous regulatory connections between pre-mRNA splicing and chromatin have been demonstrated, the precise mechanisms by which chromatin factors influence spliceosome assembly and/or catalysis remain unclear. To probe the genetic network of pre-mRNA splicing in the fission yeast Schizosaccharomyces pombe, we constructed an epistatic mini-array profile (E-MAP) and discovered many new connections between chromatin and splicing. Notably, the nucleosome remodeler SWI/SNF had strong genetic interactions with components of the U2 snRNP SF3 complex. Overexpression of SF3 components in SWI/SNF cells led to inefficient splicing of many fission yeast introns, predominantly those with non-consensus splice sites. Deletion of SWI/SNF decreased recruitment of the splicing ATPase Prp2, suggesting that SWI/SNF promotes co-transcriptional spliceosome assembly prior to first step catalysis. Importantly, defects in SWI/SNF as well as SF3 overexpression each altered nucleosome occupancy along intron-containing genes, illustrating that the chromatin landscape both affects--and is affected by--co-transcriptional splicing.

published proceedings

  • PLoS Genet

altmetric score

  • 1.5

author list (cited authors)

  • Patrick, K. L., Ryan, C. J., Xu, J., Lipp, J. J., Nissen, K. E., Roguev, A., ... Guthrie, C.

citation count

  • 21

complete list of authors

  • Patrick, Kristin L||Ryan, Colm J||Xu, Jiewei||Lipp, Jesse J||Nissen, Kelly E||Roguev, Assen||Shales, Michael||Krogan, Nevan J||Guthrie, Christine

editor list (cited editors)

  • Bentley, D.

publication date

  • March 2015