Mouse hepatitis virus ORF 2a is expressed in the cytosol of infected mouse fibroblasts. Academic Article uri icon


  • A 266-bp fragment of cDNA from within gene B, ORF 2a, of MHV-A59 was used to construct a vector encoding a bacterial/viral fusion protein. Antiserum raised against this fusion protein specifically immunoprecipitates a 30K protein from infected 17Cl-1 mouse fibroblasts. The protein is localized primarily in the cytosol and not in the membranes. This is consistent with its predicted sequence and potential role as an RNA binding protein.

published proceedings

  • Virology

author list (cited authors)

  • Zoltick, P. W., Leibowitz, J. L., Oleszak, E. L., & Weiss, S. R.

citation count

  • 18

complete list of authors

  • Zoltick, PW||Leibowitz, JL||Oleszak, EL||Weiss, SR

publication date

  • February 1990