The ns 4 gene of mouse hepatitis virus (MHV), strain A 59 contains two ORFs and thus differs from ns 4 of the JHM and S strains. Academic Article

abstract

• The sequence of the MHV-A 59 non-structural gene 4 (ns 4) reveals two open reading frames. The upstream ORF potentially encodes a 19 amino acid (2.2 kDa) polypeptide and the downstream ORF potentially encodes a 106 amino acid (11.7 kDa) polypeptide. This is in contrast to MHV-JHM gene 4 which expresses a 15 kDa protein. Cell free translation of a synthetic mRNA containing both ORFs of MHV-A 59 ns 4 results in the synthesis of a 2.2 kDa polypeptide; the predicted 11.7 kDa product of the MHV-A 59 downstream ORF is not detected during cell free translation nor in infected cells. These results add to the recent data suggesting that expression of some of the ns proteins of MHV is not necessary for efficient growth in tissue culture.

authors

• Leibowitz, Julian

published proceedings

• Arch Virol

author list (cited authors)

• Weiss, S. R., Zoltick, P. W., & Leibowitz, J. L.

citation count

• 32

complete list of authors

• Weiss, SR||Zoltick, PW||Leibowitz, JL

publication date

• March 1993

publisher

• Springer Nature  Publisher

published in

• Archives of Virology  Journal

keywords

• Amino Acid Sequence
• Base Sequence
• Cell-Free System
• Cells, Cultured
• DNA, Viral
• Genes, Viral
• Molecular Sequence Data
• Murine Hepatitis Virus
• Open Reading Frames
• Species Specificity
• Viral Nonstructural Proteins

Digital Object Identifier (DOI)

• 10.1007/BF01316905

start page

• 301

end page

• 309

volume

• 129

issue

• 1-4

URL

• http://dx.doi.org/10.1007/bf01316905