In vitro growth of two related leporipoxviruses in lymphoid cells. Academic Article

abstract

• We examined the in vitro growth patterns of two leporipoxviruses, malignant rabbit fibroma virus (MV) and Shope fibroma virus (SFV), in lymphoid cells. MV replicates well in normal spleen cells in vitro. At low m.o.i. (0.001), dramatic virus growth occurs in unstimulated cell cultures. This growth is enhanced by addition of the T lymphocyte mitogen, concanavilin A, or the B lymphocyte mitogen, Escherichia coli lipopolysaccharide. Shope fibroma virus does not grow in lymphocytes in culture, with or without mitogen stimulation. MV itself profoundly inhibits lymphocyte mitogenesis, while SFV does not. MV and SFV added to normal lymphocytes do not appear to alter their viability in culture. Thus, MV appears to be novel in its ability to replicate to high titer in resting lymphocytes. This growth pattern may be useful in understanding MV-induced immunologic dysfunction.

authors

• Leibowitz, Julian

published proceedings

• Virology

author list (cited authors)

• Strayer, D. S., Skaletsky, E., & Leibowitz, J. L.

citation count

• 14

complete list of authors

• Strayer, DS||Skaletsky, E||Leibowitz, JL

publication date

• September 1985

publisher

• Elsevier  Publisher

published in

• Virology  Journal

keywords

• Animals
• Cell Survival
• Cells, Cultured
• Female
• Fibroma Virus, Rabbit
• Kinetics
• Lymphocytes
• Poxviridae
• Rabbits
• Spleen
• Vaccinia Virus

PubMed Central ID

• 2992155

Digital Object Identifier (DOI)

• 10.1016/0042-6822(85)90167-9

start page

• 330

end page

• 334

volume

• 145

issue

• 2

URL

• http://dx.doi.org/10.1016/0042-6822(85)90167-9