Association of mouse fibrinogen-like protein with murine hepatitis virus-induced prothrombinase activity. Academic Article

abstract

• Previously, we demonstrated induction of a unique macrophage prothrombinase during infection of BALB/cJ mice by mouse hepatitis virus strain 3 (MHV-3). By immunologic screening, a clone representing this prothrombinase was isolated from a cDNA library and sequenced. The sequence identified this clone as representing part of a gene, musfiblp, that encodes a fibrinogen-like protein. Six additional clones were isolated, and one clone, p11-3-1, encompassed the entire coding region of musfiblp. Murine macrophages did not constitutively express musfiblp but, when infected with MHV-3, synthesized musfiblp-specific mRNA. musfiblp mRNA induction was earlier and significantly greater in BALB/cJ than A/J macrophages. Prothrombinase activity was demonstrated when musfiblp was expressed from p11-3-1 in RAW 264.7 cells. These data suggest that musfiblp encodes the MHV-induced prothrombinase.

authors

• Leibowitz, Julian

published proceedings

• J Virol

author list (cited authors)

• Parr, R. L., Fung, L., Reneker, J., Myers-Mason, N., Leibowitz, J. L., & Levy, G.

citation count

• 61

complete list of authors

• Parr, RL||Fung, L||Reneker, J||Myers-Mason, N||Leibowitz, JL||Levy, G

publication date

• August 1995

publisher

• American Society for Microbiology  Publisher

published in

• Journal of Virology  Journal

keywords

• Amino Acid Sequence
• Animals
• Base Sequence
• Blotting, Northern
• Cells, Cultured
• DNA, Viral
• Enzyme Induction
• Fibrinogen
• Mice
• Mice, Inbred BALB C
• Molecular Sequence Data
• Murine Hepatitis Virus
• RNA, Messenger
• Thromboplastin
• Transfection

PubMed Central ID

• 7609073

Digital Object Identifier (DOI)

• 10.1128/JVI.69.8.5033-5038.1995

start page

• 5033

end page

• 5038

volume

• 69

issue

• 8

URL

• http://dx.doi.org/10.1128/jvi.69.8.5033-5038.1995