Association of mouse fibrinogen-like protein with murine hepatitis virus-induced prothrombinase activity. Academic Article uri icon


  • Previously, we demonstrated induction of a unique macrophage prothrombinase during infection of BALB/cJ mice by mouse hepatitis virus strain 3 (MHV-3). By immunologic screening, a clone representing this prothrombinase was isolated from a cDNA library and sequenced. The sequence identified this clone as representing part of a gene, musfiblp, that encodes a fibrinogen-like protein. Six additional clones were isolated, and one clone, p11-3-1, encompassed the entire coding region of musfiblp. Murine macrophages did not constitutively express musfiblp but, when infected with MHV-3, synthesized musfiblp-specific mRNA. musfiblp mRNA induction was earlier and significantly greater in BALB/cJ than A/J macrophages. Prothrombinase activity was demonstrated when musfiblp was expressed from p11-3-1 in RAW 264.7 cells. These data suggest that musfiblp encodes the MHV-induced prothrombinase.

published proceedings

  • J Virol

author list (cited authors)

  • Parr, R. L., Fung, L., Reneker, J., Myers-Mason, N., Leibowitz, J. L., & Levy, G.

citation count

  • 61

complete list of authors

  • Parr, RL||Fung, L||Reneker, J||Myers-Mason, N||Leibowitz, JL||Levy, G

publication date

  • August 1995