A storable encapsulated bilayer chip containing a single protein nanopore. Academic Article uri icon


  • A robust, portable chip containing a single protein nanopore would be a significant development in the practical application of stochastic sensing technology. Here, we describe a chip in which a single alpha-hemolysin (alphaHL) pore in a planar phospholipid bilayer is sandwiched between two layers of agarose gel. These encapsulated nanopore chips remain functional after storage for weeks. The detection of the second messenger inositol 1,4,5-trisphosphate (IP3) was demonstrated with a chip containing a genetically engineered alphaHL pore as the sensor element.

published proceedings

  • J Am Chem Soc

altmetric score

  • 9

author list (cited authors)

  • Kang, X., Cheley, S., Rice-Ficht, A. C., & Bayley, H.

citation count

  • 122

complete list of authors

  • Kang, Xiao-feng||Cheley, Stephen||Rice-Ficht, Allison C||Bayley, Hagan

publication date

  • April 2007