Circadian vasopressin release from perifused rat suprachiasmatic explants in vitro: effects of acute stimulation. Academic Article

abstract

• Using a perifusion culture technique, the pattern of vasopressin release from the suprachiasmatic nucleus was studied in vitro under basal and stimulated conditions. Individual rat suprachiasmatic explants released vasopressin in a rhythmic fashion and these rhythms persisted with a circadian period for up to 4 cycles. One-hour exposures to KCl on days 2 and 4 in culture induced acute increases in vasopressin output and altered the circadian pattern of release from perifused suprachiasmatic explants. These results provide evidence that the release of vasopressin in an acute as well as a circadian fashion from isolated suprachiasmatic neurons is indicative of active release processes. Furthermore, it is anticipated that this method of studying circadian rhythms generated by suprachiasmatic explants may provide a basis for elucidating the neural organization of mammalian circadian pacemakers.

authors

• Earnest, David

published proceedings

• Brain Res

author list (cited authors)

• Earnest, D. J., & Sladek, C. D.

citation count

• 73

complete list of authors

• Earnest, DJ||Sladek, CD

publication date

• October 1987

publisher

• Elsevier  Publisher

published in

• BRAIN RESEARCH  Journal

keywords

• Animals
• Circadian Rhythm
• Kinetics
• Male
• Organ Culture Techniques
• Potassium Chloride
• Rats
• Rats, Inbred Strains
• Suprachiasmatic Nucleus
• Vasopressins

Digital Object Identifier (DOI)

• 10.1016/0006-8993(87)90952-8

start page

• 398

end page

• 402

volume

• 422

issue

• 2

URL

• http://dx.doi.org/10.1016/0006-8993(87)90952-8