Identification of a secreted superoxide dismutase in Mycobacterium avium ssp. paratuberculosis Academic Article uri icon

abstract

  • Mycobacterium avium ssp. paratuberculosis (M. paratuberculosis), the causative agent of Johne's disease, is an important animal pathogen that has also been implicated in human disease. The major proteins expressed by M. paratuberculosis were analyzed by two-dimensional gel electrophoresis, and a superoxide dismutase (Sod) was identified from this protein profile. The M. paratuberculosis Sod has a molecular mass of 23 kDa and an isoelectric point of 6.1. Sequence analysis of the corresponding sodA gene from M. paratuberculosis indicates that this protein is a manganese-dependent enzyme. We show that the M. paratuberculosis Sod is actively secreted, suggesting that it may elicit a protective cellular immune response in the host during infection.

author list (cited authors)

  • Liu, X., Feng, Z., Harris, N. B., Cirillo, J. D., Bercovier, H., & Barletta, R. G.

citation count

  • 33

publication date

  • August 2001