Two-component protein hydrogels assembled using an engineered disulfide-forming protein-ligand pair. Academic Article uri icon


  • We present the development of a two-component self-assembling protein hydrogel. The building blocks of the hydrogel are two liquid-phase protein block copolymers each containing (1) a subunit of the trimeric protein CutA as a cross-linker and (2) one member of a PDZ-domain-containing protein-ligand pair whose interaction was reinforced by an engineered disulfide linkage. Mixing of the two building blocks reconstitutes a self-assembling polypeptide unit, triggering hydrogel formation. This hydrogel exhibits extremely high solution stability at neutral and acidic pHs and in a wide range of temperatures (4-50 C). Incorporation of a "docking station peptide" binding motif into a hydrogel building block enables functionalization of the hydrogel with target proteins tagged with a "docking protein". We demonstrated the application of an enzyme-functionalized hydrogel in a direct electron transfer enzymatic biocathode. These disulfide-reinforced protein hydrogels provide a potential new material for diverse applications including industrial biocatalysis, biosynthesis, biofuels, tissue engineering, and controlled drug delivery.

published proceedings

  • Biomacromolecules

author list (cited authors)

  • Guan, D., Ramirez, M., Shao, L., Jacobsen, D., Barrera, I., Lutkenhaus, J., & Chen, Z.

citation count

  • 29

complete list of authors

  • Guan, Dongli||Ramirez, Miguel||Shao, Lin||Jacobsen, Daniel||Barrera, Ivan||Lutkenhaus, Jodie||Chen, Zhilei

publication date

  • August 2013