Two-component protein hydrogels assembled using an engineered disulfide-forming protein-ligand pair.
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We present the development of a two-component self-assembling protein hydrogel. The building blocks of the hydrogel are two liquid-phase protein block copolymers each containing (1) a subunit of the trimeric protein CutA as a cross-linker and (2) one member of a PDZ-domain-containing protein-ligand pair whose interaction was reinforced by an engineered disulfide linkage. Mixing of the two building blocks reconstitutes a self-assembling polypeptide unit, triggering hydrogel formation. This hydrogel exhibits extremely high solution stability at neutral and acidic pHs and in a wide range of temperatures (4-50 C). Incorporation of a "docking station peptide" binding motif into a hydrogel building block enables functionalization of the hydrogel with target proteins tagged with a "docking protein". We demonstrated the application of an enzyme-functionalized hydrogel in a direct electron transfer enzymatic biocathode. These disulfide-reinforced protein hydrogels provide a potential new material for diverse applications including industrial biocatalysis, biosynthesis, biofuels, tissue engineering, and controlled drug delivery.
author list (cited authors)
Guan, D., Ramirez, M., Shao, L., Jacobsen, D., Barrera, I., Lutkenhaus, J., & Chen, Z.
complete list of authors
Guan, Dongli||Ramirez, Miguel||Shao, Lin||Jacobsen, Daniel||Barrera, Ivan||Lutkenhaus, Jodie||Chen, Zhilei