Evaluation study of a portable impedance biosensor for detection of avian influenza virus. Academic Article uri icon

abstract

  • Current methods for detection of avian influenza virus (AIV) based on virus culture and RT-PCR are well established, but they are either time consuming or require specialized laboratory facilities and highly trained technicians. A simple, rapid, robust, and reliable test, suitable for use in the field or at the patient's bedside, is urgently needed. In this study, the performance of a newly developed portable impedance biosensor was evaluated by comparison with real-time reverse transcriptase PCR (rRT-PCR) and virus culture for detection of AIV in tracheal and cloacal swab samples collected from experimentally H5N2 AIV infected chickens. The impedance biosensor system was based on a combination of magnetic nanobeads, which were coated with AIV subtype-specific antibody for capture (separation and concentration) of a target virus, and a microfluidic chip with an interdigitated array microelectrode for transfer and detection of target virus, and impedance measurement of the bio-nanobeads and AI virus complexes in a buffer solution. A comparison of results obtained from 59 swab samples using virus culture, impedance biosensor and rRT-PCR methods showed that the impedance biosensor technique was comparable in sensitivity and specificity to rRT-PCR. Detection time for the impedance biosensor is less than 1h.

published proceedings

  • J Virol Methods

altmetric score

  • 1

author list (cited authors)

  • Wang, R., Lin, J., Lassiter, K., Srinivasan, B., Lin, L., Lu, H., ... Li, Y.

citation count

  • 37

complete list of authors

  • Wang, Ronghui||Lin, Jianhan||Lassiter, Kentu||Srinivasan, Balaji||Lin, Lin||Lu, Huaguang||Tung, Steve||Hargis, Billy||Bottje, Walter||Berghman, Luc||Li, Yanbin

publication date

  • December 2011