Expression of an Nkx3.1-CRE gene using ROSA26 reporter mice. Academic Article

abstract

• The genetic locus of Nkx3.1, an early murine marker of sclerotome and prostate development, was disrupted by a knock in of CRE recombinase via homologous recombination in embryonic stem cells. Cell fate mapping revealed previously unidentified cell lineages expanded from Nkx3.1-expressing cell populations and recapitulated reported Nkx3.1 expression patterns. In lineage trace experiments of E18.5 Nkx3.1-CRE; R26R embryos novel staining was observed in areas of the lungs, portions of the duodenum, and vertebral elements of the skeleton. beta-galactosidase activity measured in Nkx3.1-CRE; R26R and Nkx3.2-CRE; R26R embryos was observed in overlapping regions of the sclerotome but no apparent change in Nkx3.1 expression was seen in the Nkx3.2 mutants by in situ hybridization.

authors

• Zimmer, Warren

published proceedings

• Genesis

author list (cited authors)

• Stanfel, M. N., Moses, K. A., Carson, J. A., Zimmer, D. B., DeMayo, F., Schwartz, R. J., & Zimmer, W. E.

citation count

• 16

complete list of authors

• Stanfel, MN||Moses, KA||Carson, JA||Zimmer, DB||DeMayo, F||Schwartz, RJ||Zimmer, WE

publication date

• November 2006

publisher

• Wiley  Publisher

published in

• Genesis: The Journal of Genetics and Development  Journal

keywords

• Animals
• Beta-Galactosidase
• Cell Differentiation
• Cell Lineage
• DNA Primers
• Duodenum
• Embryonic Stem Cells
• Gene Expression Profiling
• Gene Expression Regulation, Developmental
• Homeodomain Proteins
• In Situ Hybridization
• Lung
• Mice
• Spine
• Transcription Factors

PubMed Central ID

• 17078065

Digital Object Identifier (DOI)

• 10.1002/dvg.20250

start page

• 550

end page

• 555

volume

• 44

issue

• 11

URL

• http://dx.doi.org/10.1002/dvg.20250