Intramuscular delivery of a cholera DNA vaccine primes both systemic and mucosal protective antibody responses against cholera. uri icon

abstract

  • Cholera is a potentially lethal diarrhea disease caused by the gram-negative bacterium Vibrio cholerae. The need for an effective cholera vaccine is clearly indicated but the challenges of eliciting both systemic and mucosal immune responses remains a significant challenge. In the current report, we discovered that a DNA vaccine expressing a protective cholera antigen, cholera toxin B subunit (CTB), delivered parenterally can elicit both systemic and mucosal anti-CTB antibody responses in mice. The priming effect by DNA immunization was demonstrated by higher mucosal antibody responses following one boost with the inactivated cholera vaccine (KWC-B) delivered orally when compared to the twice oral administration of KWC-B alone. This finding indicates that DNA vaccines delivered parenterally are effective in eliciting mucosal protective immune responses--a unique advantage for DNA vaccination that has not yet been well realized and should bring value to the development of novel vaccination approaches against mucosally transmitted diseases.

published proceedings

  • Vaccine

author list (cited authors)

  • Xu, G., Wang, S., Zhuang, L., Hackett, A., Gu, L., Zhang, L. u., ... Lu, S.

complete list of authors

  • Xu, Guifang||Wang, Shixia||Zhuang, Ling||Hackett, Anthony||Gu, Ling||Zhang, Lu||Zhang, Chunhua||Wang, Hua||Huang, Zuhu||Lu, Shan

publication date

  • June 2009