Detection of Tick-Borne Pathogens by MassTag Polymerase Chain Reaction Academic Article uri icon

abstract

  • MassTag polymerase chain reaction (PCR) is a platform that enables microbe detection using primers labeled through a photocleavable link with tags that vary in molecular weight. After multiplex PCR, tags are released by ultraviolet irradiation and analyzed by mass spectroscopy. The identification of a microbe in a sample is determined by its cognate tags. Here we describe establishment and implementation of a MassTag PCR panel for surveillance of microbes implicated in tick-vectored infectious diseases.

author list (cited authors)

  • Tokarz, R., Kapoor, V., Samuel, J. E., Bouyer, D. H., Briese, T., & Lipkin, W. I.

citation count

  • 25

publication date

  • April 2009