Detection of tick-borne pathogens by MassTag polymerase chain reaction. Academic Article uri icon

abstract

  • MassTag polymerase chain reaction (PCR) is a platform that enables microbe detection using primers labeled through a photocleavable link with tags that vary in molecular weight. After multiplex PCR, tags are released by ultraviolet irradiation and analyzed by mass spectroscopy. The identification of a microbe in a sample is determined by its cognate tags. Here we describe establishment and implementation of a MassTag PCR panel for surveillance of microbes implicated in tick-vectored infectious diseases.

published proceedings

  • Vector Borne Zoonotic Dis

author list (cited authors)

  • Tokarz, R., Kapoor, V., Samuel, J. E., Bouyer, D. H., Briese, T., & Lipkin, W. I.

citation count

  • 27

complete list of authors

  • Tokarz, Rafal||Kapoor, Vishal||Samuel, James E||Bouyer, Donald H||Briese, Thomas||Lipkin, W Ian

publication date

  • April 2009