Detection of Tick-Borne Pathogens by MassTag Polymerase Chain Reaction
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MassTag polymerase chain reaction (PCR) is a platform that enables microbe detection using primers labeled through a photocleavable link with tags that vary in molecular weight. After multiplex PCR, tags are released by ultraviolet irradiation and analyzed by mass spectroscopy. The identification of a microbe in a sample is determined by its cognate tags. Here we describe establishment and implementation of a MassTag PCR panel for surveillance of microbes implicated in tick-vectored infectious diseases.
author list (cited authors)
Tokarz, R., Kapoor, V., Samuel, J. E., Bouyer, D. H., Briese, T., & Lipkin, W. I.