Detection of tick-borne pathogens by MassTag polymerase chain reaction. Academic Article

abstract

• MassTag polymerase chain reaction (PCR) is a platform that enables microbe detection using primers labeled through a photocleavable link with tags that vary in molecular weight. After multiplex PCR, tags are released by ultraviolet irradiation and analyzed by mass spectroscopy. The identification of a microbe in a sample is determined by its cognate tags. Here we describe establishment and implementation of a MassTag PCR panel for surveillance of microbes implicated in tick-vectored infectious diseases.

authors

• Samuel, James

published proceedings

• Vector Borne Zoonotic Dis

author list (cited authors)

• Tokarz, R., Kapoor, V., Samuel, J. E., Bouyer, D. H., Briese, T., & Lipkin, W. I.

citation count

• 29

complete list of authors

• Tokarz, Rafal||Kapoor, Vishal||Samuel, James E||Bouyer, Donald H||Briese, Thomas||Lipkin, W Ian

publisher

• Mary Ann Liebert  Publisher

published in

• Vector-Borne and Zoonotic Diseases  Journal

keywords

• Anaplasma
• Animals
• Arachnid Vectors
• Borrelia
• DNA Primers
• Polymerase Chain Reaction
• Ticks
• United States

Digital Object Identifier (DOI)

• 10.1089/vbz.2008.0088

start page

• 147

end page

• 152

volume

• 9

issue

• 2

URL

• http://dx.doi.org/10.1089/vbz.2008.0088