Effect of cargo size and shape on the transport efficiency of the bacterial Tat translocase. Academic Article

abstract

• The Tat machinery translocates fully-folded and oligomeric substrates. The passage of large, bulky cargos across an ion-tight membrane suggests the need to match pore and cargo size, and therefore that Tat transport efficiency may depend on both cargo size and shape. A series of cargos of different sizes and shapes were generated using the natural Tat substrate pre-SufI as a base. Four (of 17) cargos transported with significant (>20% of wild-type) efficiencies. These results indicate that cargo size and shape significantly influence Tat transportability.

authors

• Musser, Siegfried

published proceedings

• FEBS Lett

author list (cited authors)

• Whitaker, N., Bageshwar, U., & Musser, S. M.

citation count

• 3

complete list of authors

• Whitaker, Neal||Bageshwar, Umesh||Musser, Siegfried M

publication date

• April 2013

publisher

• Wiley  Publisher

published in

• FEBS Letters  Journal

keywords

• Biological Transport
• Blotting, Western
• Cell Membrane
• Cysteine
• Escherichia Coli
• Escherichia Coli Proteins
• Green Fluorescent Proteins
• Kinetics
• Luminescent Measurements
• Membrane Transport Proteins
• Models, Molecular
• Molecular Weight
• Mutation
• Protein Conformation
• Protein Precursors

PubMed Central ID

• 23422074

Digital Object Identifier (DOI)

• 10.1016/j.febslet.2013.02.015

start page

• 912

end page

• 916

volume

• 587

issue

• 7

URL

• http://dx.doi.org/10.1016/j.febslet.2013.02.015