Effect of cargo size and shape on the transport efficiency of the bacterial Tat translocase Academic Article uri icon


  • The Tat machinery translocates fully-folded and oligomeric substrates. The passage of large, bulky cargos across an ion-tight membrane suggests the need to match pore and cargo size, and therefore that Tat transport efficiency may depend on both cargo size and shape. A series of cargos of different sizes and shapes were generated using the natural Tat substrate pre-SufI as a base. Four (of 17) cargos transported with significant (>20% of wild-type) efficiencies. These results indicate that cargo size and shape significantly influence Tat transportability.

author list (cited authors)

  • Whitaker, N., Bageshwar, U., & Musser, S. M.

citation count

  • 2

publication date

  • February 2013