The role of fibroblast growth factor (FGF) in neoplasms induced by MoMuSV-349.
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abstract
Previous results from our laboratory have demonstrated that intraperitoneal inoculation of newborn BALB/c mice with MoMuSV-349 virus induced multiorgan disseminated angiomatous tumors. Changes in histological pattern, from sarcomatous to angiosarcomatous, stimulated our interest in investigating the possible role of angiogenic growth factors released by the spindled (sarcomatous) cells in angiosarcoma development in this model. Cell lines were obtained from various tumors and assayed for production of acidic (a) and basic (b) fibroblast growth factors (FGFs). All tumor cell lines released detectable amounts of growth factor(s) into the cultured media that induced proliferation of endothelial cells and mitogenesis of mouse fibroblasts. This growth factor(s) bound to heparin Sepharose (HS) beads and its effects on cell proliferation were partially blocked by a neutralizing bFGF antibody. Proteins released by tumor cells into the conditioned medium were detected by bFGF antibodies on Western blots. In addition, proteins that reacted with both bFGF and aFGF antibodies were detected in various conditioned media by ELISA. This protein(s) from the FGF family detected in the conditioned media from various tumor cell lines might be responsible for the angiomatous proliferation observed in the late (angiosarcomatous) stage of MoMuSV-induced tumors.
