Analysis of phosphorylated isoforms of the p53 tumor suppressor protein in human lung carcinoma cells undergoing apoptosis. Academic Article

abstract

• The expression of p53 tumor suppressor protein isoforms in H460a cells induced to undergo apoptosis by 2-methoxyestradiol was investigated by two-dimensional gel electrophoresis. Whole-cell proteins from control and apoptotic H460a cells were separated by two-dimensional electrophoresis and were transferred to nitrocellulose. The p53 isoforms were detected by immunoblotting using p53 monoclonal antibody Bp53-12. Four isoforms of p53 (2, 3, 5, and 6) differing in phosphorylation state were detected in control cells. Three additional isoforms (1, 4, and 7) were observed to be expressed at significant levels only in apoptotic cells. The differential expression of isoforms 1, 4, and 7 in apoptotic cells suggests that one or more specific phosphorylation events generating these forms of p53 could play a role in regulating the function of p53 in apoptosis.

authors

• Maxwell, Steven

published proceedings

• Electrophoresis

author list (cited authors)

• Maxwell, S. A., Roth, J. A., & Mukhopadhyay, T.

citation count

• 16

complete list of authors

• Maxwell, SA||Roth, JA||Mukhopadhyay, T

publication date

• November 1996

publisher

• Wiley  Publisher

published in

• Electrophoresis  Journal

keywords

• 2-methoxyestradiol
• Apoptosis
• Blotting, Western
• Carcinoma, Non-Small-Cell Lung
• DNA Fragmentation
• Electrophoresis, Gel, Two-Dimensional
• Estradiol
• Gene Expression Regulation, Neoplastic
• Humans
• Lung Neoplasms
• Neoplasm Proteins
• Phosphoproteins
• Phosphorylation
• Protein Processing, Post-Translational
• Tumor Cells, Cultured
• Tumor Suppressor Protein P53

PubMed Central ID

• 8982610

Digital Object Identifier (DOI)

• 10.1002/elps.1150171115

start page

• 1772

end page

• 1775

volume

• 17

issue

• 11

URL

• http://dx.doi.org/10.1002/elps.1150171115